Scotland looked capable of producing a shock when they drew level through Callum McGregor but were ultimately beaten by the better side.
Portugal enjoyed their previous European Championship meeting with France, but that was a rare victory against Les Bleus.
Germany and Hungary are each still in contention at Euro 2020 after recovering from damaging matchday one defeats.
Scotland's first major tournament appearance in 23 years was brought to an end by Croatia at Hampden Park.
Livewire teenager Bukayo Saka says England believe they can beat anybody at Wembley in the last 16 of Euro 2020.
Arsenal teenager Bukayo Saka was a surprise selection for England against the Czech Republic and responded with a man-of-the-match display.
Group D came to a conclusion on Tuesday as England, Croatia and the Czech Republic all progressed – so Scotland miss out.
Sweden have not thrilled the neutral at Euro 2020, but Poland coach Paulo Sousa considers them "perfect".
Raheem Sterling can frustrate at times but he has shown himself to be invaluable, lifting a stuttering England into the knockout stages.
With the danger of results elsewhere going against them, Spain must beat Slovakia on matchday three in Group E.
Away goals will no longer be a factor in knockout ties after UEFA announced a major change to the rules moving forward.
The Netherlands have won their three group games at a European Championship for only the third time as they scored an easy in on Monday.
Kevin De Bruyne "did not think about" the injury he sustained in the Champions League final when he came on to lift Belgium against Denmark.
Milan were unable to agree a new deal with Hakan Calhanoglu and the attacking midfielder has now crossed the divide by joining Inter.
Kacper Kozlowski became the youngest ever Euros player just six days after Jude Bellingham had that honour.
Roberto Mancini knows Italy will face a different tournament heading into the knockout stages, as Aaron Ramsey celebrated Wales' progress.
Bayern Munich "would have liked it" if UEFA had approved Germany's request to support the LGBTQ+ community with rainbow lights.
Former Real Madrid team-mates Cristiano Ronaldo and Karim Benzema starred as the Euro 2020 group stage came to an end.
Captain Luka Modric says Croatia must finally click into gear when they face Scotland in a must-win encounter on Tuesday.